Views: 0 Author: Site Editor Publish Time: 2023-03-04 Origin: Site
Choose Jinteng economical HPLC syringe filters to remove particulates from your liquid samples prior to injection on your HPLC. Our syringe filters are economical and designed to be single-use for your convenience and for laboratory efficiency. When choosing a syringe filter, three main factors dictate your syringe filter selection: sample volume, potential particulate size, and filter membrane compatibility.
Diameter of the Syringe Filter and Sample Volume
Sample volume dictates the diameter of the syringe filter. Intuitively, larger diameter syringe filters (e.g., 25mm syringe filters) filter larger sample sizes because of their wide cross-sectional membrane area, which offers increased filtration speed over the smaller diameter filters.Jinteng 25mm syringe filters can be chosen for filter samples 10-15mL.
13mm syringe filters are a popular choice for most chromatography methods and typically chosen when sample volume is less than 10mL.
Syringe Filter Pore Size
Two porosities of membranes are available for our syringe filters, 0.45 micron and 0.2micron. The 0.45 micron pore is recommended to protect most HPLC instrumentation and columns from detrimental particulates. The newer trend is for chromatographers to develop assays with lower levels of detection, which in turn leads to columns of smaller particle size and the HPLC tubing internal diameter to get smaller and smaller. This trend to go smaller means that chromatographers need to filter their samples to remove even smaller particles; therefore, in these scenarios, it is recommended to use the 0.2 micron pore size syringe filter.
In one words,if the micron size of column is less than 3mm,the pore size0.2micron is suitable；while if the micron size of column is more than3mm,the pore size 0.45micro is best choice.
Syringe Filter Membrane Options
Jinteng stocks the most common membrane types to cover a wide range of applications. The main selection criteria for your syringe filter membrane is solvent compatibility and application (specifically, if you are concerned with protein binding).
Nylon membranes are used for general laboratory filtration of HPLC samples prior to injection. Nylon is solvent resistant, exhibits lows extractables, and is compatible with both aqueous and organic solvents. Nylon binds protein and should not be used when protein recovery is important. Nylon syringe filters have a green color-coded ring around the housing body for easy identification.
PTFE is treated by ethanol or isopropyl become into a hydrophilic membrane,which can be natural moist.Resistant to strong acid,strong alkali organic solvent and antioxidant. PTFE syringe filters have a pink or orange color-coded ring around the housing body for easy identification.
PVDF is also treated by ethanol or isopropyl become into a hydrophilic membrane,which is a solvent-resistant membrane that exhibits low levels of UV-adsorbing extractables and is recommended for HPLC sample filtration and general biological filtration. PVDF is a low-protein binding membrane and compatible with both aqueous and organic solvents. PVDF syringe filters have a light green or purple color-coded ring around the housing body for easy identification.
PES (polyethersulfone) is a mechanically strong membrane low in inorganic extractable ions. Typical applications include ion chromatography, tissue culture filtration, and filtration of proteins and nucleic acids. PES is a low-protein binding membrane and compatible with both aqueous and organic solvents. PES syringe filters have a yellow or blue color-coded ring around the housing body for easy identification.
